A detailed view on 1,8-cineol biosynthesis by Streptomyces clavuligerus

نویسندگان

  • Jan Rinkel
  • Patrick Rabe
  • Laura zur Horst
  • Jeroen S Dickschat
چکیده

The stereochemical course of the cyclisation reaction catalysed by the bacterial 1,8-cineol synthase from Streptomyces clavuligerus was investigated using stereospecifically deuterated substrates. In contrast to the well investigated plant enzyme from Salvia officinalis, the reaction proceeds via (S)-linalyl diphosphate and the (S)-terpinyl cation, while the final cyclisation reaction is in both cases a syn addition, as could be shown by incubation of (2-13C)geranyl diphosphate in deuterium oxide.

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عنوان ژورنال:

دوره 12  شماره 

صفحات  -

تاریخ انتشار 2016